Resources

As a provider of quality embryo transfer services, our goals include informing you of all
aspects. Part of our commitment to the agricultural community is to equip and educate
you on current issues.

These guidelines have been developed based on the experience of our veterinarian team
interacting with clients to help avoid oversights and achieve success in applying embryo
transfer

Training in Embryology

We provide training in embryo transfer services, from donor and recipient management
and synchronization to the superovulation and flushing of donors. We also offer training
in all aspects of embryo handling, including certifying embryos for international export,
sexing and freezing embryos.

 

Embryo Thawing Protocols

All export embryos are frozen following IETS guidelines and importing country protocols for embryo handling. All our embryos are frozen in ethylene glycol and labeled DT for direct transfer. Direct transfer is additionally reinforced by use of yellow plugs and storage in yellow goblets with yellow cane tops.

Unique cane identification consists of the donor dam short name, breeding sire short name and the date of flush on the cane top.

Paperwork accompanying the embryos clearly identifies the embryo, and should be checked before thawing.

Maximize embryo pregnancy rates:

I. Recipient management:

  • Recipient management is very important.
  • Recipients should be well grown for their age and be gaining weight on a balanced diet.
  • Recipients should have an observed standing heat 7 days prior.
  • Examine the recipient rectally for uterine and ovarian conditions consistent with her intended use as a recipient that day.

II. Thawing direct transfer embryos:

The use of ethylene glycol has simplified embryo thawing with consistently good pregnancy rates.

Cryoprotectant dilution is not required. Do not re-evaluate the embryo microscopically post-thaw. This procedure is not recommended as it increases the time between thaw and transfer, which should be kept to no more than 10 minutes. Extra steps increase the risk of pregnancy failures. 

Consult ET paperwork for each embryo prior to thawing.

  1. Examine and prepare the recipient before thawing embryos to check her suitability to receive an embryo.
    • Ovarian structures are to be consistent with an observed standing heat 7 days prior. 
    • The uterus must be healthy, capable of a high conception rate. 
    • If the recipient is to receive an embryo, administer an epidural. 
  2. Remove one straw from the cane/goblet and immediately place in a 35-38⁰C warm water bath for 15 - 20 seconds. Dry the straw with a clean paper towel. This will allow you to grasp the straw easily to firmly twist and pull the label plug out. Retain the label plug for identification purposes. Avoid touching the open end of the straw. 
  3. Load the straw. We use long Cassou/IMV E.T. guns; 3mm for heifers, and 4.1mm for cows. Ensure that the sheath fits snugly at the end of the ET gun when loaded. Trim the straw at the cotton plug end if the straw is too long. 
  4. Transfer to the uterine horn on the same side as the corpus luteum. All embryos that are thawed must be transferred. Transfer embryos within 10 minutes of removing from liquid nitrogen.
  5. Carefully identify recipient and embryo ID for record keeping.

Biosecurity

About Us

The Process

Embryos

Sexing and IVF Technologies

Resources